Bacterial Chemotaxis and Biodegradation of p-nitrophenol/4-nitrocatechol: Molecular and Biochemical Studies
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Description
The work presented in here mainly involves biochemical and molecular studies on bacterial degradation of p-nitrophenol and its degradation products. It also focuses on the study of biodiversity of a p-nitrophenol contaminated soil and the diversity and phylogeny of benzenetriol dioxygenase, an enzyme involved in p-nitrophenol degradation. p-Nitrophenol is known to be degraded via two pathways by bacteria. In the first case p-nitrophenol is degraded via the formation of 4-nitrocatechol and 1,2,4-benzenetriol, while the other pathway proceeds through the formation of benzoquinone and hydroquinone. However, there are only few reports related to the gene(s) involved in degradation and their regulation. Attempts were made to elucidate and characterize some of the gene(s) involved in p-nitrophenol degradation in the laboratory and some of the gene(s) of the lower degradation pathway encoded on a ~6.8 kb fragment of the genomic DNA of strain SJ98 were cloned in pBluescript vector.