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Description
An efficient protocol providing a dual regeneration pathway via shoot organogenesis and somatic embryogenesis for an endangered species, Metabriggsia ovalifolia W. T. Wang, was established from leaf explants. When applied at 2.5 M, the cytokinins 6-benzyladenine or thidiazuron and the auxins indole-3-butyric acid, α-naphthaleneacetic acid and indole-3-acetic acid could induce shoots when on basal Murashige and Skoog medium. BA and TDZ at 5-10 M could induce both shoots and somatic embryos. A higher concentration (25 M) of TDZ induced only somatic embryos.